Journal: Nature immunology

Article Title: Cytotoxic innate lymphoid cells sense cancer cell-expressed interleukin-15 to suppress human and murine malignancies.

doi: 10.1038/s41590-022-01213-2

Figure Lengend Snippet: Fig. 1 | chRCC and ccRCC tumors exhibit differential immune cell infiltration and CD8 T cell phenotypes. a, t-Distributed stochastic neighbor embedding (tSNE) of transcriptional profiles from leukocytes isolated from one chRCC tumor and one ccRCC tumor. Each dot represents a single CD45+ cell, and colors represent clusters denoted by cell type inferred from lineage markers and differential gene expression. b, tSNE plot as in a, colored by histology (chromophobe or clear cell). c, For each CD8 cluster, the frequency out of all CD8α+ clusters at which it was found in chRCC (n = 1) and ccRCC (n = 1) tumors. d, Violin plots showing log-normalized expression of selected differentially expressed genes (DEGs) among three CD8 clusters. e, Representative plots of flow cytometric analysis of the percentage of CD3+CD8α+ T cells out of the lymphocyte gate (CD45+SSCLow) in blood, adjacent normal kidney and tumor samples from one patient of the indicated histology. Quantification is CD3+CD8α+ T cells out of total CD45+ cells. f, Representative histograms of PD-1 expression in CD3+CD8α+ T cells from blood, adjacent normal kidney and tumor tissues from a single patient of the indicated histology. Quantification of flow cytometric analysis of percentage of PD-1+CD3+CD8α+ T cells in blood, adjacent normal kidney and tumor samples from the indicated histology. e,f, Each pair of symbols connected by a line denotes an individual patient (chRCC blood n = 6 (e and f), kidney n = 9 (e) and 10 (f), tumor n = 9 (e) and 10 (f); ccRCC blood n = 14, kidney n = 15 and tumor n = 16). One-way analysis of variance (ANOVA) with Tukey’s multiple comparison test was used for statistical analysis (NS, not significant; **P < 0.01, ****P < 0.0001.

Article Snippet: Antihuman CD8a (RPA-T8, #25-0088-T100) and biotinylated anti-human CD3 (UCHT1, #30-0038-U100) were purchased from Tonbo Biosciences.

Techniques: Isolation, Gene Expression, Expressing, Comparison

Journal: Aging (Albany NY)

Article Title: EGFR-specific CAR-T cells trigger cell lysis in EGFR-positive TNBC

doi: 10.18632/aging.102510

Figure Lengend Snippet: Characterization of T lymphocytes from PBMCs. ( A – B ) T cell phenotypes and subsets were examined by flow cytometry after labeling with anti-CD3-PE-Cy7, anti-CD4-PE, and anti-CD8-APC-Cy7.

Article Snippet: Anti-Human CD3 (PE-Cy7), anti-Human CD4 (PE), and anti-Human CD8 (APC-Cy7) antibodies, as well as corresponding mouse IgG controls, were purchased from BioGems.

Techniques: Flow Cytometry, Labeling

Journal: iScience

Article Title: Hypoxic-immune axis orchestrates metastatic dissemination via HIF isoform imbalance in pancreatic neuroendocrine tumors

doi: 10.1016/j.isci.2025.114340

Figure Lengend Snippet: Immune cell infiltration in KRAS G12C mutant PNETs (A) Quantitative analysis using Image Plus 6.0 software reveals a significant increase in Tregs (CD4 + , CD25 + , and FoxP3 + ) within KRAS- G12C mutant PNETs compared to wild-type KRAS PNET tissues, suggesting KRAS G12C -driven immunosuppressive cell recruitment. (B) Fluorescence intensity analysis demonstrates reduced infiltration of CD8 + cytotoxic T cells and HLA-DR + activated cells in KRAS G12C mutant tumors, indicative of impaired antitumor immunity. (C) Elevated MDSCs counts in KRAS G12C mutant PNETs correlate with enhanced immune evasion.

Article Snippet: APC Anti-Human CD8a Antibody [OKT-8] , Elabscience , E-AB-F1110E.

Techniques: Mutagenesis, Software, Fluorescence

Journal: iScience

Article Title: Hypoxic-immune axis orchestrates metastatic dissemination via HIF isoform imbalance in pancreatic neuroendocrine tumors

doi: 10.1016/j.isci.2025.114340

Figure Lengend Snippet: Tregs, CD8 + T cells, and HLA-DR + cells in KRAS G12C -mutated PNETs (A) Flow cytometry plots and fluorescence intensity histograms demonstrate elevated CD4 + T cell proportions in KRAS G12C patient blood samples compared to wild-type KRAS tumors and healthy controls. (B) Quantification shows a significant enrichment of CD25 + T cells in KRAS G12C patients, surpassing both wild-type KRAS tumors and normal controls. (C) Quantitative data and histogram overlays confirm a substantial increase in FoxP3 + T cells frequency in KRAS G12C patients, with levels moderately elevated compared to wild-type KRAS and significantly higher than healthy individuals. (D) A slight decrease in CD8 + T cell frequency in KRAS G12C samples relative to wild-type KRAS, with levels significantly lower than those in healthy individuals (E) A moderate reduction in HLA-DR + cell frequency in KRAS G12C patients compared to wild-type KRAS, alongside a marked suppression relative to healthy controls.

Article Snippet: APC Anti-Human CD8a Antibody [OKT-8] , Elabscience , E-AB-F1110E.

Techniques: Flow Cytometry, Fluorescence